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LT2 Monitoring

LT2 Monitoring

The Long Term 2 Enhanced Surface Water Treatment Rule (LT2ESWTR or LT2 rule) addresses the health effects associated with Cryptosporidium in surface water used as a drinking water supply. All public water systems using surface water or groundwater under the direct influence of surface water are required to monitor under LT2.

EMSL Qualifications:

  • EMSL is fully approved by the USEPA for LT2 Round 2 Cryptosporidium monitoring program using EPA method 1623/1623.1.
  • EMSL is cerified in most states to analyze LT2 samples.
  • EMSL is a leading laboratory with the largest sample capacity for LT2 testing.
  • EMSL surpassed other laboratories participating in the most recent EPA’s performance testing (PT) studies with 72.5% to 68.0% for recoveries of Cryptosporidium (national average is 59.5%).

Frequently Asked Questions:

  • Can you take samples from my state?

    We can take Cryptosporidium samples from most states. Please contact Aquatic Sciences Laboratory for our capability to analyze Cryptostropridium in your state.

  • When should I start sampling for Round 2?

    Please contact your state for the specific dates. Below is a general sampling schedule for Public Water Suppliers (PWSs).


System Serving Population

Begin Date

Tests to perform

Schedule 1


April 1, 2015


E. coli, Turbidity

Schedule 2

50,000 - 99,000

Oct. 1, 2015


E. coli, Turbidity

Schedule 3

10,000 - 49,999

Oct. 1, 2016


E. coli, Turbidity

Schedule 4

(E. Coli)


Oct. 1, 2017

E. coli

Schedule 4



April 1, 2019


  • How do I collect samples for testing?

    EMSL can accept either 10 L bulk water or field filtered samples. For field filtered samples, EMSL offers a crypto filtration apparatus to easily and accurately collect sample on field. EMSL will provide a detailed sampling instruction.

  • How to ship samples?

    Generally, all crypto samples should be shipped overnight. EMSL also offer pick up services by our couriers, which depends on your location. Please contact Aquatic Sciences Laboratory for the possibility to pick up samples from your location.


Atlanta, GA (LAB 07) - NVLAP Lab Code 101048-1Baton Rouge, LA (LAB 25) - NVLAP Lab Code 200375-0Beltsville, MD (LAB 19) - NVLAP Lab Code 200293-0Boston, MA (LAB 13) - NVLAP Lab Code 101147-0Buffalo, NY (LAB 14) - NVLAP Lab Code 200056-0Carle Place, NY (LAB 06) - NVLAP Lab Code 101048-10Charlotte, NC (LAB 41) - NVLAP Lab Code 200841-0Chicago, IL (LAB 26) - NVLAP Lab Code 200399-0Cinnaminson, NJ (LAB List in Description) - NVLAP Lab Code 101048-0Dallas, TX (LAB 11) - NVLAP Lab Code 600111-0Denver, CO (LAB 22) - NVLAP Lab Code 200828-0EMSL Canada - Calgary, AB (LAB 65) - NVLAP Lab Code 500100-0EMSL Canada - Edmonton, AB (LAB 50) - NVLAP Lab Code 600321-0EMSL Canada - Markham, ON (LAB 66) - NVLAP Lab Code 600317-0EMSL Canada - Montreal, QC (LAB 68) - NVLAP Lab Code 201052-0EMSL Canada - Ottawa, ON (LAB 67) - NVLAP Lab Code 201040-0EMSL Canada - Toronto, ON (LAB 55) - NVLAP Lab Code 200877-0EMSL Canada - Vancouver, BC (LAB 69) - NVLAP Lab Code 201068-0Fort Lauderdale, FL (LAB 56) - NVLAP Lab Code 500085-0Houston, TX (LAB 15) - NVLAP Lab Code 102106-0Huntington Beach, CA (LAB 33) - NVLAP Lab Code 101384-0Indianapolis, IN (LAB 16) - NVLAP Lab Code 200188-0Kernersville, NC (LAB 02) - NVLAP Lab Code 102104-0Las Vegas, NV (LAB 31) - NVLAP Lab Code 600140-0Long Island City, NY (LAB 03) - NVLAP Lab Code 101048-9Meriden, CT (LAB 24) - NVLAP Lab Code 200700-0Miami, FL (LAB 17) - NVLAP Lab Code 200204-0Minneapolis, MN (LAB 35) - NVLAP Lab Code 200019-0Ontario, California (San Bernadino County / Inland Empire) (LAB 71) - NVLAP Lab Code 600239-0Orlando, FL (LAB 34) - NVLAP Lab Code 101151-0Phoenix, AZ (LAB 12) - NVLAP Lab Code 200811-0Piscataway, NJ (LAB 05) - NVLAP Lab Code 101048-2Plymouth Meeting, PA (LAB 18) - NVLAP Lab Code 200699-0Raleigh, NC (LAB 29) - NVLAP Lab Code 200671-0Rochester, NY (LAB 53) - NVLAP Lab Code 600183-0San Diego, CA (LAB 43) - NVLAP Lab Code 200855-0San Leandro, CA (LAB 09) - NVLAP Lab Code 101048-3Santa Clara, CA (LAB 47) - NVLAP Lab Code 600318-0Seattle, WA (LAB 51) - NVLAP Lab Code 200613-0South Pasadena, CA (LAB 32) - NVLAP Lab Code 200232-0South Portland, ME (LAB 62) - NVLAP Lab Code 500094-0St. Louis, MO (LAB 39) - NVLAP Lab Code 200742-0Syracuse, NY (LAB 73) - NVLAP Lab Code 600283-0Tampa, FL (LAB 93) - NVLAP Lab Code 600215-0
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