In recent years, bed bugs (Cimex lectularius) have become a big concern for homeowners and hotel travelers. The increasing activities of bed bugs are related in part to the changes in pesticide usage and global travel. A rapid qPCR method for detecting bed bugs has been developed at EMSL Analytical, Inc. We were able to find the presence of bed bugs by analyzing samples from bedroom dust, swabs from headboards, around beds, and mattresses; and air samples.