E. coli O157:H7 is one of the most pathogenic strains of E. coli. Undercooked or raw hamburger (ground beef) has been implicated in many of the documented outbreaks, however E. coliO157:H7 outbreaks have also originated from contaminated alfalfa sprouts, unpasteurized fruit juices, dry-cured salami, lettuce, game meat, and cheese curds. Raw milk was the vehicle in a school outbreak in Canada.
This method detects the Presence or Absence of the bacterium. A confirmation charge may be incurred in cases of Presumptive Positive screens.
50g (750g for 375g testing) minimum sample is recommended. Environmental surface samples can also be analyzed.
EMSL Test Codes:
- Vidas (ECPT AOAC RI 060903): F108 (Environmental), F233 (25g), F129 (375g)
- BAX (AOAC RI 031002): F189 (Environmental), F194 (25g), F195 (375g)
- FDA BAM (FDA BAM Ch. 4A): F232
Sample retention time – 30 days, unless highly perishable